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Registros recuperados: 5
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Cloning and characterization of newly isolated lipase from Enterobacter sp. Bn12 BJM
Farrokh,Parisa; Yakhchali,Bagher; Asghar Karkhane,Ali.
A mesophilic Enterobacter sp. Bn12 producing an alkaline thermostable lipase was isolated from soil in Tehran, Iran. The lipase gene (ELBn12) was identified from a genomic library. Sequence analysis of the DNA fragment revealed an open reading frame of 879 bp encoding a lipase with a molecular mass of 31.3 kDa. The deduced amino acid sequence showed 96% identity with a lipase of Enterobacter sp. Ag1 and the identity of their DNA sequences was 88.9%. ELBn12 belongs to the lipase subfamily I.1 and its catalytic triad consists of Ser82, Asp237 and His259. The lipase was expressed in Escherichia coli (BL21) pLysS and partially purified by anion exchange chromatography. The maximum activity of ELBn12 was obtained at temperature of 60 °C and pH 8.0 towards...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacter; Thermostable lipase; Alkaline; Organic solvent.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200042
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Draft genome sequence of Exiguobacterium aurantiacum strain PN47 isolate from saline ponds, known as “Salar del Huasco”, located in the Altiplano in the North of Chile BJM
Strahsburger,Erwin; Zapata,Felipe; Pedroso,Inti; Fuentes,Derie; Tapia,Paz; Ponce,Raul; Valdes,Jorge.
ABSTRACT In this report, we present a draft genome of 2,886,173 bp of an Exiguobacterium aurantiacum strain PN47 isolate from the sediment of a saline pond named “Salar del Huasco” in the Altiplano in the North of Chile. Strain PN47 encodes adaptive characteristics enabling survival in extreme environmental conditions of high heavy metal and salt concentrations and high alkalinity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline; Arsenic; Heavy metals; Pigments.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100007
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Microbial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the Prony Bay (New Caledonia) over a 6-year period ArchiMer
Postec, Anne; Quemeneur, Marianne; Bes, Meline; Mei, Nan; Benaissa, Fatma; Payri, Claude; Pelletier, Bernard; Monnin, Christophe; Guentas-dombrowsky, Linda; Ollivier, Bernard; Gerard, Emmanuelle; Pisapia, Celine; Gerard, Martine; Menez, Benedicte; Erauso, Gael.
Active carbonate chimneys from the shallow marine serpentinizing Prony Hydrothermal Field were sampled 3 times over a 6 years period at site ST09. Archaeal and bacterial communities composition was investigated using PCR-based methods (clone libraries, Denaturating Gel Gradient Electrophoresis, quantitative PCR) targeting 16S rRNA genes, methyl coenzyme M reductase A and dissimilatory sulfite reductase subunit B genes. Methanosarcinales (Euryarchaeota) and Thaumarchaea were the main archaeal members. The Methanosarcinales, also observed by epifluorescent microscopy and FISH, consisted of two phylotypes that were previously solely detected in two other serpentinitzing ecosystems (The Cedars and Lost City Hydrothermal Field). Surprisingly, members of the...
Tipo: Text Palavras-chave: Hydrothermal; Serpentinization; Alkaline; Prony; Microbial communities.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00467/57916/60307.pdf
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Odour Reduction from Pig Housing using Alkaline Air Cleaning CIGR Journal
Joergensen, M..
Testing the addition of an alkali (NaOH) in an air cleaner (Munters A/S) connected to a finisher pig unit during the summer resulted in an average odour reduction of 47%. The measurement days were spread over two batches of pigs (summer and autumn). The average odour reduction for the entire test period was 41%. The measurements taken during the autumn did not show a statistically significant odour reduction. Furthermore, the results showed that it was possible to reduce the emission of hydrogen sulphide by 53%. As expected, the levels of ammonia and carbon dioxide were not reduced. When using 100% air cleaning, the consumption of the air cleaner for two batches of finishers (30 – 110 kg) amounted to 355 L water and 2.4 kg 27.7% NaOH solution,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Odour; Alkaline; Air cleaner; Pig units; Denmark.
Ano: 2015 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/3166
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Odour Reduction from Pig Housing using Alkaline Air Cleaning CIGR Journal
M. Joergensen.
Testing the addition of an alkali (NaOH) in an air cleaner (Munters A/S) connected to a finisher pig unit during the summer resulted in an average odour reduction of 47%. The measurement days were spread over two batches of pigs (summer and autumn). The average odour reduction for the entire test period was 41%. The measurements taken during the autumn did not show a statistically significant odour reduction. Furthermore, the results showed that it was possible to reduce the emission of hydrogen sulphide by 53%. As expected, the levels of ammonia and carbon dioxide were not reduced. When using 100% air cleaning, the consumption of the air cleaner for two batches of finishers (30 – 110 kg) amounted to 355 L water and 2.4 kg 27.7% NaOH solution,...
Palavras-chave: Odour; Alkaline; Air cleaner; Pig units; Denmark.
Ano: 2015 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/3166
Registros recuperados: 5
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